Pathogen Gene Expression Profiling During Infection Using a Nanostring...
NanoString nCounter is a recently developed platform that can make direct multiplexed measurement of gene expression using color-coded probe pairs (Geiss et al., Nat Biotechnol 26(3):317–325, 2008;...
View ArticleDevelopment of Impala-Based Transposon Systems for Gene Tagging in...
Genome sequences of many filamentous fungi are now available and additional genomes are currently being sequenced. One of the next strategic goals is to generate collections of tagged genes in order to...
View ArticleChromatin Immunoprecipitation Analysis in Filamentous Fungi
Chromatin immunoprecipitation (ChIP) is used to map the interaction between proteins and DNA at a specific genomic locus in the living cell. The protein–DNA complexes are stabilized already in vivo by...
View ArticleProtoplast Transformation of Filamentous Fungi
The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged mutations into the...
View ArticleTargeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens-...
Genome sequence data on fungal pathogens provide the opportunity to carry out a reverse genetics approach to uncover gene function. Efficient methods for targeted genome modifications such as knockout...
View ArticleCell Fusion in the Filamentous Fungus, Neurospora crassa
Hyphal fusion occurs at different stages in the vegetative and sexual life cycle of filamentous fungi. Similar to cell fusion in other organisms, the process of hyphal fusion requires cell recognition,...
View ArticleDNA Fingerprinting Candida Species
It is sometimes necessary to assess the genetic relatedness of isolates to identify the origin of an infection. In addition, evidence is accumulating that drug resistance can be associated with strains...
View ArticleUsing Non-homologous End-Joining-Deficient Strains for Functional Gene...
Fungal strains deficient in the non-homologous end-joining (NHEJ) pathway are excellent recipient strains for gene targeting approaches. In addition, NHEJ-deficiency can facilitate the formation of...
View ArticleSYBR safeTM efficiently replaces ethidium bromide in Aspergillus fumigatus...
Invasive aspergillosis is a disease responsible for high mortality rates, caused mainly by Aspergillus fumigatus. The available drugs are limited and this disease continues to occur at an unacceptable...
View ArticleNorthern blot
Profile and functional analysis of small RNAs derived from Aspergillus fumigatus infected with double-stranded RNA mycovirusesNorthern blotting was used to validate the presence of vsiRNAs. Briefly, 2...
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